CHEMICAL LINKERS SWITCH TRIGLYCEROL DETERGENTS FROM BACTERIAL PROTEIN PURIFICATION TO MILD ANTIBIOTIC AMPLIFICATION

Chemical linkers switch triglycerol detergents from bacterial protein purification to mild antibiotic amplification

Abstract Non-ionic detergents enable the investigation of cell membranes, including biomolecule purification and Vacuum Filter Container drug delivery.The question of whether non-ionic detergents associated with satisfying protein yields following extraction and affinity purification of proteins from lysed E.coli membranes can amplify antibiotics o

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Luminescence Dating of Rock Surface. The Case of Monoliths from the Megalithic Sanctuary of Ossimo-Pat (Valle Camonica, Italy)

Ossimo-Pat megalithic sanctuary (Valle Camonica, BS, Italy) is one of the most relevant archaeological findings of the southern alpine region, for the variety of its structures and the quality of its engraved monoliths.Its unique HARVEST MOON CONDI state of preservation gives the opportunity to apply the luminescence dating of the rock surface meth

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Two-Step Activation Mechanism of the ClpB Disaggregase for Sequential Substrate Threading by the Main ATPase Motor

Summary: AAA+ proteins form asymmetric hexameric rings that hydrolyze ATP and thread substrate proteins through a central channel via mobile substrate-binding pore loops.Understanding how ATPase and threading activities are regulated and intertwined is key to understanding the AAA+ protein mechanism.We studied the disaggregase ClpB, which contains

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